Staphylococcus Genotyping Array
The results to the left are from a microarray that contains seven hundred signature oligos that identify and discriminate between Staphylococcus aureus strains. Panel A and B represent the same data rotated to highlight the three-dimensional aspects of the presentation. The array was designed using chromosomal and plasmid sequences from ten Staphylococcus aureus strains, some of which are susceptible to antibiotics and others that are resistant to one or more antibiotics. Unique and functional elements such as antibiotic resistance genes and virulence factors are represented. The array was hybridized with labeled fragments of genomic DNA from three strains of Staphylococcus aureus, only one of which has been sequenced. The sequenced strain (Mu50) hybridized to signature genomic and plasmid sequences as expected. The other two strains demonstrated unique hybridization patterns that had partial overlap with multiple sequenced Staphylococcus aureus strains.
Each data point is a function of its hybridization intensity in each of three separate hybridizations with labeled DNA from each of the three target organisms. Sequences present in only one organism demonstrate high hybridization intensities along a single axis. Sequences present in two of the three organisms fall on the walls of the graph because they have high intensities along two axes. Sequences present in all three organisms float in the center near the apex of the graph and are conserved in almost all of the sequenced Staph strains. Both of the unsequenced Staph strains, Wood 46, and SA113 demonstrated unique hybridization patterns. These results demonstrate the ability to rapidly generate unique sequence-based hybridization patterns for closely related organisms whose genomes have not been sequenced.
Each data point is a function of its hybridization intensity in each of three separate hybridizations with labeled DNA from each of the three target organisms. Sequences present in only one organism demonstrate high hybridization intensities along a single axis. Sequences present in two of the three organisms fall on the walls of the graph because they have high intensities along two axes. Sequences present in all three organisms float in the center near the apex of the graph and are conserved in almost all of the sequenced Staph strains. Both of the unsequenced Staph strains, Wood 46, and SA113 demonstrated unique hybridization patterns. These results demonstrate the ability to rapidly generate unique sequence-based hybridization patterns for closely related organisms whose genomes have not been sequenced.
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Panel A
Panel B
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