Real Time PCR with GenArraytion Probes

Using GenArraytion primers and fluors, real time PCR was performed to demonstrate a highly specific and highly sensitive association between a mecA probe and target mecA gene.

A log dilution of Methicillin Resistant Staphylococcus aureus was made to test the assay's limit of detection. The sensitivity of MRSA for one of our mecA markers is given in the graph above. Our assay is sensitive up to 10fg of DNA. 1ng of Escherichia coli K12 and 1ng of Human DNA were used as negative control.